L-asparaginase activity in plasma and other biological fluids

Comparative Study of L-asparaginase Activity in Different Bacterial Species

L-asparaginase exerts anti-leukemia activity

This study aimed to investigate the effects of L-Asparaginase (L-ASP) on leukemia cell proliferation, cell cycle distribution and geminin expression. Jurkat cells were treated with different concentration of LASP for 24 h, 48 h or 72 h. Cell proliferation was analysed by the CCK-8 assay, cell cycle distribution was examined by flow cytometry, and mRNA and protein expression levels of geminin we...

The Determination of Ammonia in Blood and Other Biological Fluids

In 1912 Folin and Denis (1) published a series of methods for the analysis of blood and urine, all based on the principle of Nesslerization, and among those met.hods was one for the determination of ammonia in blood. By the help of this method Folin and Denis proved the then important point t.hat the relatively high ammonia content of portal blood is mostly putrefactive ammonia absorbed from th...

L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...